哈尔滨地区发热患者风疹病毒的分离鉴定

Separation and identification of Rubella virus in febrile patients in Harbin

目的为了明确哈尔滨地区风疹病毒(R ube lla v irus,RV)的流行情况,对2003年上半年采集的部分发热患者血清及血细胞进行筛选、鉴定、比较,进行血清学和病原学研究。同时从风疹病毒IgM抗体阳性患者血中分离病毒,对疑似风疹病毒的分离株进行鉴定。方法采用酶联免疫吸附试验(EL ISA)对159例发热患者血清中的风疹病毒IgM进行测定;采用BHK 21细胞培养法对风疹病毒IgM抗体阳性患者血标本进行风疹病毒分离,并用RT-PCR、细胞生长曲线、电镜观察、免疫组化等方法对可疑风疹病毒株进行鉴定。结果风疹病毒IgM抗体阳性为22例,占13.83%;经RT-PCR、细胞生长曲线、电镜观察、免疫组化等方法初步鉴定从血液标本中获得的病毒分离株是风疹病毒。结论采用EL ISA法对该地区159例发热患者血清风疹病毒IgM进行检测,阳性率为13.83%;获得了1株风疹病毒分离株。

Objective To know the epidemic condition of Rubella virus （RV） in Harbin, we screened,evaluated and compared febrile patients＇s blood serums and blood cells in the first half year 2003,and take serumal and pathogen research. At the same time Separate RV from RV IgM positive cases,and identify doubtful RV strain. Methods In this study,we detected the RV IgM of 159 febrile cases by using ELISA; With the method of BHK21 ,we separated the RV from positive sample and identified the RV by using RT-PCR,curve of cell growth, electron microscope,immunity. Results The number of RV IgM positive cases was 22 and the positive percent was 13.83 % ;After using the methods of RT-PCR,curve of cell growth ,electron microscope ,immunity,we preliminary examine the virus separated from blood sample was RV. Conclusion The positive percent of 159 febrile cases was 13.83% by using ELISA; We attained a RV strain.