乙肝患者血清肝纤维化标志物与HBV DNA含量分析

Analysing the relation between hepatic fibrosis and hepatitis B virus DNA in serum of hepatitis B patients

目的动态观察肝纤维化活动性与肝脏病变程度。方法采用放射免疫法检测血清中透明质酸(HA)、层连蛋白(IN)、Ⅲ型前胶原(PC-Ⅲ)、Ⅳ型胶原(Ⅳ-C)肝纤维化标志物;采用核扩增荧光定量检测HBVDNA。结果随着年龄的不同血清肝纤维化标志物水平变化呈多样性,而各年龄组间的病毒含量差异无显著性(P>0.05)。结论肝炎患者HBV复制活跃,传染性强,但并不表明肝炎后肝病的严重程度。

Objective To study the relation between hepatic fibrosis activity and the degree of liver dama ge. Methods The hyaluronic acid （HA） ,procollagen type Ⅲ（PC- Ⅲ ） ,laminin（LN） ,and type Ⅳ collagen （ Ⅳ -C） in the serum was detected by RIA kit. The quantity of HBV DNA was detected by fluorescence polymerase chain reaction（PCR）. Result Hepatic fibrosis was changed with different age,while there was no difference with the quantity of HBV DNA. Conclusion Even HBV DNA had a high replication and contaminating in the patients,but it don＇t associate with the damage of liver.