骨材料矿化中两种基质囊泡的研究

Study on Two Types of Mineral-related Matrix Vesicles in the Bone Mineralization of Zebrafish

骨是一种复杂的天然复合材料。我们在斑马鱼的脊椎骨中发现了2种与矿化有关的基质囊泡：单囊泡和多囊泡。对这2种基质囊泡进行了TEM和SED分析，发现都含有钙、磷离子，说明它们可能与斑马鱼骨矿化中的离子输送有关。单囊泡与已有研究中发现的基质囊泡一样，直径为100—500nm；但多囊泡直径为700～1000nm，并由直径平均100nm的小囊泡构成。

Bone refers to a family of natural composite materials, which have highly complex components and hierarchical structures. Two types of mineral-related matrix vesicles, multivesicular body （MVB） and monovesicle, are detected in the skeletal bone of zebrafish. Transmission electron microscopy and energy dispersive spectroscopy （EDS） analyses of the vesicular inclusions reveal that both types of vesicles contain calcium and phosphorus, suggesting that these vesicles may involve in mineral ions delivery for bone mineralization of zebrafish. However, their size and substructure are quite different Monovesicles,whose diameters range from 100 - 550nm,are similar to normal matrix vesicles previously reported, while MVBs have a larger size of 700 - 1000nm in nominal diameter and possess substructure that is composed of smaller vesicles with their average size around 100nm.