+Gz对大鼠血浆和肾脏内皮素含量的影响

Effect of ＋Gz on plasma and renal endothelin content in rats

目的为了解＋Gz暴露对血浆及肾脏内皮素（ET－1）含量的影响。方法将48只雄性Wistar大鼠随机分成A、B、C、D4组（n＝12）。A：地面未受离心机＋Gi暴露；B：＋1Gz；C、D：＋10Gz。每次暴露3min，共3次，每次间隔40min。B、C组在＋Gz暴露后10min，D组在＋Gz暴露后24h，留取血液及肾组织标本。用放射免疫法测定血浆和肾脏匀浆中的ET－1含量。结果＋10Gz暴露组较＋1Gz暴露组血浆ET-1含量增加，但未获统计学支持。肾组织ET－1含量明显下降（p＜0．01）。＋10Gz暴露后24h肾脏ET-1含量恢复到＋1Gz水平。结论＋Gz暴露后，大鼠肾组织ET－1含量下降，血浆ET－1含量增加不明显。

Objective To explore the effect of +Gz stress on plasma and renal endothelin con-tent. Metbods Forty-eight Wistar rats were randomly divided into 4 groups: group A served asground control. Rats of group B were exposed to + 1 Gz and those of group C and D were exposed to+ 10 Gz 3 times (3 min each, with 40 min interval in between). Plasrna and renal tissue samples ofgroup B and C were taken 10min after +Gz exposure, while those of group D were taken 24 h after ex-posure. Plasma and renal endothelin content were measured by radioimmunoassay. Results PlasmaET-1 content of group C was higher than that of group B, but statistically nonsignificant, while renalET-1 content of group C was significantly lower than that of B. Plasrna ET-1 content of group D resti-tuted to group A level,and renal ET-1 content to group B level. Conclusion Renal ET-1 content de-creased significantly after +Gz exposures, while the increase of plasma ET-1 content was nonsignifi-cant.