旋毛虫肌肉期幼虫分泌排泄物中46-58kD蛋白的纯化及其在诊断中的应用

PURIFICATION AND APPLICATION OF 46-58 kD PROTEINS FROM EXCRETORY/SECRETORY PRODUCTS OF TRICHINELLA SPIRALIS MUSCLE LARVAE FOR DIAGNOSIS OF TRICHINOSIS WITH DOT-ELISA

本研究以常规ＳＤＳ－ＰＡＧＥ和电洗脱技术相结合，分离纯化旅毛虫肌肉期幼虫分泌排泄物（ＴＳＬＩ－ＥＳ）中４６－５８ｋＤ的蛋白组份，并用于Ｄｏｔ－ＥＬＩＳＡ诊断旋毛虫病．检测结果：旋毛虫病人血清３４份，阳性３２份，阳性反应率为９４．１２％；正常人血精１００份未发现假阳性；对蛔虫病人（１２份）、囊虫病人（１５份）、血吸虫病人（１５份）及肺吸虫病人（２０份）血清亦无交叉叵应出现；建立的Ｄｏｔ－ＥＬＩＳＡ易于操作．重复性和稳定性较好。用Ｄｏｔ－ＥＬＩＳＡ比较纯化抗原和ＴｓＬ１－ＥＳ的诊断效果，结果经卡方检验后显示：用纯化抗原和用ＴｓＬ１－ＥＳ检测的敏感性无显著差异，但纯化抗原的特异性显著优于ＴｓＬ１－ＥＳ。

The 46-58 kD proteins from excretory/secretory products of Trichinella spiralis muscle larvae(TslL1-ES) were seperated and purified by common SDS-PAGE and electroelutiontechnique, and applied for diagnosing trichinosis with Dot-ELISA. The results showedthat the positive rate was 94. 12% (32/34) in cases of trichinosis, but no false positive andcross reaction were found in sera from 100 normal individuals, 12 sera of ascariasis, 15sera of cysticercosis, 15 sera of schistosomiasis and 20 sera of paragonimiasis. The purifiedantigens were compared with that of TsL1-ES by Dot-ELISA. There was no significantdifference in sensitivity between the two antigens but in contrast, the specificity of purifiedantigens was significantly higher than that of TsL1-ES.