Identification of potential biomarkers of Peyronie's disease

瞄准：为了识别是的蛋白质，在房间表示的差别作为与 Peyronie 的疾病(PD ) 有关源于正常和 diseased 大桶集成通信适配器白膜(TA ) 。方法：有成纤维细胞的特征的房间从二织物来源被孤立。当从一样的病人的通常出现的 TA 的磅房间，和那些被指定为 NT 房间，那些从有 PD 的病人的匾被指明。这些房间的送信人 RNA 被即时聚合酶链反应(RT-PCR ) 为单核白血球 chemoattractant 蛋白质 1 的表示(MCP-1 ) 分析。粗略的蛋白质 lysates 被提高表面的激光解吸附作用 / 电离团分光术(SELDIMS ) 与 IMAC30-Cu， CM10，和 H50 薄片分析。每溶解产物然后被分开成六部分，它被 SELDIMS 进一步分析。结果：RT- PCR 分析证明磅房间比他们的对应物 NT 房间表示了 MCP-1 的高水平。SELDIMS 分析证明所有四房间紧张的粗略的蛋白质 lysates 在 IMAC30-Cu 和 CM10 薄片上生产了类似、可再现的蛋白质侧面。有 fractionated lysates 的另外的 SELDIMS 分析检测了 11.6 kDa 的三蛋白质， 14.5 kDa，起来的 22.6 kDa 在磅房间和低的 kDa 和 46.9 kDa 在磅房间调整了的 6.3 的二蛋白质调整了。结论：MCP-1，经常涉及织物纤维变性，在 NT 房间比那在磅在高水平被表示。为 PD 的五个潜在的简历标记被 SELDIMS 分析识别。

Aim： To identity proteins that are differentially expressed in cells derived from normal and diseased tunica albuginea （TA） as related to Peyronie＇s disease （PD）. Methods： Cells with characteristics of fibroblasts were isolated from two tissue sources. Those from the plaque of patients with PD were designated as PT cells, and those from the normally- appearing TA of the same patients were designated as NT cells. Messenger RNAs of these cells were analyzed by real-time polymerase chain reaction （RT-PCR） for the expression of monocyte chemoattractant protein 1 （MCP-1）. Crude protein lysates were analyzed by surface-enhanced laser desorption/ionization mass spectrometry （SELDI-MS） with IMAC30-Cu, CM10, and H50 chips. Each lysate was then separated into six fractions, which were further analyzed by SELDI-MS. Results： RT- PCR analysis showed that PT cells expressed higher levels of MCP-1 than their counterpart NT cells. SELDI-MS analysis showed that the crude protein lysates of all four cell strains produced similar and reproducible protein profiles on IMAC30-Cu and CM 10 chips. Additional SELDI-MS analyses with the fractionated lysates detected three proteins of 11.6 kDa, 14.5 kDa, 22.6 kDa that were upregulated in PT cells and two proteins of 6.3 kDa and 46,9 kDa that were downregulated in PT cells. Conclusion： MCP-1, which is often involved in tissue fibrosis, was expressed at higher levels in PT than that in NT cells. Five potential biomarkers for PD were identified by SELDI-MS analysis. （Asian J Androl 2005 Sep; 7： 237-243）