膀胱移行细胞癌中Survivin基因的检测及临床意义

Detection of survivin in bladder carcinoma and its clinical significance

目的:探讨Survivin基因在膀胱移行细胞癌中的表达及其临床意义。方法:通过逆转录-聚合酶链反应(RT-PCR)检测40例膀胱癌和12例非膀胱癌组织及尿液标本中Survivin基因mRNA的表达,采用免疫组织化学方法(SP法)复检Survivin基因的表达并确定其临床病理分期和分级。结果:40例膀胱癌组织中Survivin基因的阳性表达率为67.5%,mRNA表达结果与免疫组织化学结果一致;在尿液中的阳性表达率为55.5%。12例非膀胱癌组织中Survivin基因的阳性表达率仅为8.3%;尿液中未见Survivin基因表达。显示Survivin基因在膀胱癌中的表达显著高于在非膀胱癌中的表达(P<0.05);而组织标本与尿液标本对Survivin基因的检出率无明显差异(P>0.05);Survivin的阳性表达与膀胱癌的恶性程度、临床病理分级或是否复发关系密切(P<0.05),Survivin基因的阳性表达与患者年龄、性别、肿瘤的分期无明显相关性。结论:Survivin基因在膀胱癌的恶性程度、肿瘤转移及预后等方面可能有较大的临床意义,而检测尿液中Survivin基因的表达可作为膀胱癌的早期发现、早期诊断、术后复查的新方法。

Objective：To study the expression and detection of survivin in bladder transitional cell carcinoma （BTCC） and its clinical significance. Methods：We detected the expression of survivin mRNA in tissues and exfoliated urothelial cells from 40 BTCC patients and 12 non-BTCC patients by RT-PCR. And immunohistochemistry was used to detect the expression of survivin protein and identify the pathologyical grade and clinical stage in 40 BTCC cases. Results：There was no significant difference of the rate of expression between tissue specimen and urine specimen in BTCC patients. The rate of expression between tissue specimen and urine specimen in BTCC patients was no significant difference. The positive expression Of survivin was correlated with pathological grade or relapse recurrence（ P 〈0.05） ,but not with age,sex and clinical stage（ P 〉0.05）. Conclusions：The levels of survivin are elevated in BTCC. Survivin may play an important role in BTCC grade, metastasis or prognosis. And detecting the expression of survivin in urine may be a new method to find and diagnose in early stage or reexamine after operation.