摘要
A polymerized liposome （PLS） was prepared using a synthesized phospholipid with a diacetylene moiety.The precipitable property of the PLS on salt additions,ligand immobilization,affinity adsorption kinetics and adsorption isotherms were studied.Trypsin was purified by affinity precipitation from a crude pancreatic extract.The specific activity reached about 13000 U.mg－1. These results indicate that the PLS is a potential ligand carrier for affinity precipitation of proteins.
A polymerized liposome (PLS) was prepared using a synthesized phospholipid with a diacetylene moiety.The precipitable property of the PLS on salt additions,ligand immobilization,affinity adsorption kinetics and adsorption isotherms were studied.Trypsin was purified by affinity precipitation from a crude pancreatic extract.The specific activity reached about 13000 U.mg^(－1). These results indicate that the PLS is a potential ligand carrier for affinity precipitation of proteins.
出处
《化工学报》
EI
CAS
CSCD
北大核心
1996年第2期222-227,共6页
CIESC Journal
基金
国家教委优秀年轻教师基金
关键词
聚合脂质体
亲和沉淀
吸附平衡
胰蛋白酶
纯化
polymerized liposome,affinity precipitation,adsorption equilibrium,trypsin,purification
