候选抗病基因在水稻品种多样性研究中的应用

Application of the Candidate Resistance Genes to Genetic Diversity in Rice

利用19个来源于水稻和大麦、具有抗病基因NBS-LRR结构特点的探针对24个水稻品种的DraI酶切DNA片段进行候选抗病基因分析,用26个稻瘟病菌株对供试品种进行人工接种试验。结果表明:供试品种中共检测到127个候选基因位点,平均每个探针为6.7个。除齐头谷、八宝谷和紫糯外,籼稻品种的位点数均高于粳稻品种,而这些籼稻品种表现较为抗病。聚类分析将供试品种划分为籼粳差异明显的2个类群和分群结果与其系谱关系及抗性基本吻合的5个亚群,表明候选抗病基因标记可用于水稻品种的遗传关系、水稻病害控制和抗病育种方面研究。

The homologues of 24 rice cultivars were analyzed using 19 candidate resistance gene （CRG） probes from rice and barley with NBS-LRR structure, while the resistance of the cultivars was tested by inoculating 26 isolates of Magnaporthe grisea in this study. The results showed that the 24 cultivars had 127 loci totally, each probe had 6.7 loci averagely. Except for Qitougu, Babaogu and Zinuo, indica cultivars had more loci and higher blast resistance than japonica ones in field and artificial inoculation, implying the positive correlation between the DNA band numbers and blast resistance. The clustering analysis classified the 24 cultivars into 2 groups and 5 subgroups, which respectively corresponded to indica-japonica differentiation and known rice heterotic groups based on pedigree information and blast reaction. The results demonstrated that CRG markers could be used to investigate genetic relationships among rice cultivars, and to control rice blast by inter-planting different cultivars and thus providing rice resistance breeding with parent genetic background.