CSN3、CSN1S2和β-1g基因多态与西农萨能奶山羊产羔数的相关性研究

Relationship Between Polymorphisms of CSN3,CSN1S2 and β-lg Genes and Litter Sizes of Xinong Saanen Dairy Goat

首次利用PCR-RFLP技术探讨κ酪蛋白(CSN3)、αs2酪蛋白(CSN1S2)和β-乳球蛋白(β-lg)基因多态与69只西农萨能奶山羊产羔数的相关性。结果表明:CSN3-TaqI位点与产羔数之间存在显著相关(P<0.05或P<0.01),如TC基因型个体第1胎产羔数高于CC型(P<0.01),CC基因型个体第2胎产羔数高于CC型(P<0.05);CSN3-HindIII位点与产羔数之间不存在关联(P>0.05);CSN1S2-Alw26I位点与产羔数间存在显著的相关性(P<0.05或P<0.01),如NF基因型个体第1胎产羔高于NN型(P<0.05),NN基因型个体第4胎产羔数高于NF型(P<0.01);β-lg-smaI位点与产羔数间不存在显著相关(P>0.05)。结果提示CSN3和CSN1S2基因对奶山羊产羔数有显著影响,从而推测酪蛋白基因可能与FecB基因连锁。因此,认为CSN3-TaqI和CSN1S2-Alw26I位点可作为奶山羊高产羔数标记辅助选择(MAS)的有效分子标记。

In this paper, PCR-RFLP technique was firstly adopted to analyze the association of polymorphisms of CSN3, CSNIS2 and beta-lactoglobulin genes with litter sizes of 69 Xinong Saanen dairy goats. The results revealed that there was a statistical significant relationship between CSN3-TaqⅠ locus and litter sizes （P 〈 0.05）. For example, the first birth litter size of individuals with genotype TC were more than those of Individuals with genotype CC （P 〈 0.01）, while the second birth litter size of individuals with genotype CC were more than those of individuals with genotype TC （P 〈 0.05）. Moreover, the results showed that no statistical significant difference between CSN3-HindlII locus and litter sizes was found （P 〉 0.05）. However, there was significant relationship between CSN1S2-Alw26Ⅰ locus and litter sizes. For instance, the first birth litter sizes of individuals with genotype NF more than those of genotype NN （P 〈 0.05）, while the fourth birth litter sizes of individuals with genotype NF was less than those of genotype NN （P 〈 0.01）. No significant correlation between beta-lactoglobulin gene and litter sizes was observed in this experiment, Based on these results, it was indicated that CSN3 and CSN1S2 gene had significant positive effects on litter sizes of dairy goats. So it is primitively presumed that casein gene such as CSN3, CSN1S2 gene and Fec^B gene probably might be linked, which would well explain the gene effects of CSN3 and CSN1 S2 gene on litter sizes. Additionally, CSN3 and CSNI S2 gene can be regarded as efl＇ective molecular markers on MAS program for litter size of dairy goat.