摘要
目的建立反相高效液相色谱测定地骨皮中莨菪亭含量的方法。方法使用DiamonsilC18色谱柱(4.6mm×200mm,5μm),流动相为甲醇-冰醋酸水溶液(1→100)(25:75,pH=3.0),检测波长为345nm。结果在0.0018-0.1440μg内莨菪亭呈现良好线性关系(r=0.9999)。RSD<1.3%。结论用本法测定地骨皮中莨菪亭含量灵敏准确,为该药材的质量评价提供了一定的参考依据。
OBJECTIVE develop a RP-HPLC method to determine the content of scopoletin in Cortex Lycii from different sources. METHODS The separation was accomplished on a Diamonsil C18 column (4.6 mm × 200 mm, 5μm) with a mixture of methanol- aqueous acetic acid ( 1→ 100) (25:75, pH = 3.0) as the mobile phase. The eluates were deteeted at 345 nm. RGSULTS The linear range was 0.001 8-0.144 0 μg ( r= 0.999 9). The precision was perfect (RSD〈 1.3%) .CONCLUSION This study lind adequate sensitivity and accuracy to measure the contents of scopoletin and could be used in the quality control of Cortex Lycii.
出处
《中国药学杂志》
EI
CAS
CSCD
北大核心
2005年第22期1741-1742,共2页
Chinese Pharmaceutical Journal