双黄隆反相高效液相色谱分析

Analysis of Shuanghuanglong by Reversedphase High performance Liquid Chromatography

本文报道用反相高效液相色谱分析双黄隆可湿性粉剂，其有效成分甲黄隆、绿黄隆含量的方法，以甲醇和１％乙酸钠水溶液作流动相，以香豆素为内标物，在２５４纳米下检测。甲黄隆、绿黄隆变异系数分别为０．３４％、０．６１％。

In this paper.a reversed-phase high performance liquid chromatography method to determine two active ingredients of Shuanghuanglong consisting metsulfuron-methyl and chlorsulfuron was described.Chromatographic conditions were:clolumn, Nova-Pak C18,5μ,3.9mm×150mm; mobile phase,MeOH:H2O:NaAc (30:70: 0. 7, v/v/m); internal standard , coumarin;flow rate,1.2ml/min;deteded at 254nm.The variation coefficient were 0. 34%and 0. 61%, respectively.