硫酸铜蓄积对日本蟳体内保护酶系统的影响

Effects of accumulated copper sulfate on protective system of Charybdis japonica

首先根据急性毒性试验确定了硫酸铜对日本蟳Charybdis japonica的安全浓度为0.50 mg/L,然后进行了硫酸铜蓄积对日本蟳体内5种组织保护酶系统的影响试验。结果表明:日本蟳体内的保护酶系统由超氧化物歧化酶(SOD)和过氧化氢酶(CAT)组成,在所有检测的组织中均未发现过氧化物酶(POD)的存在;各组织中SOD、CAT的酶活性显示出高度的组织特异性,SOD的酶活性依次为:心脏>鳃和肌肉>性腺>肝胰脏,CAT的酶活性依次为:性腺>肝胰脏>心脏>鳃>肌肉;日本蟳体内的保护酶在硫酸铜作用下发生了相应的变化,药物短时间胁迫能对酶产生一定的刺激作用,而长时间胁迫会抑制酶的活性;不同组织对硫酸铜的敏感性不同,鳃、肝胰脏和性腺对硫酸铜的敏感性大于心脏和肌肉的。

The acute toxicity of various concentrations of copper sulfate to Charybdis japonica was determined, and the safety concentration was 0.50 mg/L. Then, effects of 0.50 mg/L copper sulfate accumulation on protective system in muscle, gill, hepatopancreas, heart and gonad were studied. The results showed that superoxide dismutase （SOD） and catalase （CAT） activities were found to be ranged in a descendant order as the following： heart 〉 gill and muscle 〉 gonad and hepatopancreas, gonad 〉 hepatopancreas 〉 heart 〉 gill and muscle, respectively. Short time accumulation of the chemicals stimulated SOD and CAT activities, while the activities dropped during the long period. The sensitivities of SOD and CAT to the poison were much more in gill, sex gland and hepatopancreas than those in the heart and muscle.