用酶联免疫法测定虾蟹中残留的氯霉素

The application of ELISA method in determination of Chloramphenicol residues in Chinese mitten crab and white-legged shrimp (Litoppenaeus vannamei)

采用酶联免疫法测定了南美白对虾Penaeus vannamei和中华绒螯蟹Eriocheir sinensis 中残留的氯霉素.比较了用不同方法处理样品对检测结果的影响,通过对检测范围、精密度、回收率等项目的测定,验证了该方法的可靠性.结果表明:氯霉素标准液为50～4050 ng/L时,具有良好的线性关系;板间变异系数小于16.4%,样品测定的变异系数为9.4%～13.8%;用乙腈水溶液方法提取的回收率(84%～116 %)比用乙酸乙酯提取的回收率(62%～92%)高.

Enzyme- linked immunosorbent assay （ELISA） method was used to determine chloramphenicol （CAP） residues in Chinese mitten crab （Eriocheir sinensis ） and white - legged shrimp （Litopenaeus vannamei）. The results showed that different distillation method affected the testing data. The standard curve was rectilinear from the rage of 50 ng/L to 4 050 ng/L. The intra - assay variations of kits were less than 16.4%. The variations of sample ranged from 9.4% to 13.8%. The recovery varied from 84% to 116%.