旋光度测定法测定硫酸阿米卡星注射液中主药的含量

Assaying of Principal Agent in Amikacin Sulfate Injection by Polarimetry

目的:建立以旋光度测定法测定硫酸阿米卡星注射液中主药含量的方法.方法:以水为溶剂,用1dm测定管测定硫酸阿米卡星注射液在589.4nm波长下的旋光度.结果:阿米卡星检测浓度在1～20mg/ml范围内与旋光度线性关系良好(r=0.9999),平均加样回收率为99.8%(RSD=0.89%,n=5).结论:本方法简便、快速、准确,可用于本品的质量控制.

OBJECTIVE： To establish polarimetry for the assaying of the principal agent in amikacin sulfate injection. METHODS： The optical rotation of amikacin sulfate was determined with ldm determination tube with water as solvent at a wavelength of 589.4nm.RESULTS： The linear range of amikacin was 1-20mg/ml（r = 0.9 999）; The average recovery rate was 99.8% （RSD = 0.89% ,n = 5）.CONCLUSION：The method is simple,fast and accurate,and it can be used for the quality control of amikacin sulfate injection.