精乌口服液的质量标准研究

Study on Quality Standard of Jingwu Oral Liquid

目的:完善精乌口服液的质量标准。方法:采用薄层色谱法对精乌口服液中的制何首乌和黄精(制)进行定性鉴别,并采用高效液相色谱法对其中2,3,5,4’—四羟基二苯乙烯—2—O—β—D—葡萄糖苷含量进行测定。结果:精乌口服液中制何首乌和黄精(制)供试品薄层色谱中,在与对照品色谱相应的位置上,显相同颜色的斑点;2,3,5,4’—四羟基二苯乙烯—2—O—β—D—葡萄糖苷进样量在0·0562μg^0·6744μg范围内与峰面积积分值线性关系良好(r=0·9996),平均加样回收率为100·1%(RSD=1·89%)。结论:本方法回收率高、重现性好、简便、快速、准确,可用于本品的质量控制。

OBJECTIVE： To improve the quality standard for Jingwu oral liquid.METHODS： The qualitation of Radix Polygoni Multiflori preparata and solomonseal rhizome in Jingwu oral liquid were identified by TLC and the assaying of 2,3, 5, 4＇ -tetra-hydroxyl diphenyl ethylene-2-O-β-D-glucoside in the oral liquid were determined by HPLC.RESULTS： In the TLCs of Radix Polygoni Multiflori preparata and solomonseal rhizome, the color of speckles was the same as that of the control articles in the corresponding places.The linear range of 2, 3, 5,4＇-tetra-hydroxyl diphenyl ethylene-2-O-β-D- glucoside was 0.0 562μg- 0.6 744μg（ r = 0.9 996） ; The average recovery was 100.1% （RSD = 1.89 % ） . CONCLUSION ： The method is simple,fast,and reproducible and of high recovery rate,which can be used as quality control for Jingwu oral liquid.