依地酸钙钠干预急性百草枯中毒大鼠肺损伤的实验

THE STUDY ON THE EFFECTS AND MECHANISM OF EDTA ON ACUTE PARAQUAT INTOXICATION INDUCED PULMONARY INJURY

目的:拟从肺组织的病理学基础、酶学指标变化及细胞内钙离子浓度变化等方面,探讨依地酸钙钠(ED-TA)在百草枯中毒肺损伤中的作用以及其作用机制。方法:取SD大鼠78只,随机数字表法分为治疗(高、中、低剂量)组、阳性对照组及阴性对照组;给予一次性腹腔内注入百草枯20 mg/kg后,再按分组情况分别给予药物(治疗组剂量分别为10 mg/kg、20 mg/kg、40 mg/kg)或生理盐水;在6个不同时间点观察其肺病理组织改变,测量肺组织匀浆内的丙二醛(MDA)含量及超氧化物歧化酶(SOD)的活力。结果:给予EDTA后,肺泡壁充血、出血均有减轻,且高剂量组的各时期肺泡上皮细胞增生明显,易见核分裂。MDA含量下降(P<0.01);SOD活力逐渐升高(P<0.01)。结论:EDTA可减轻百草枯中毒后所引起的弥漫性肺损伤,减轻氧自由基损伤,抑制脂质过氧化作用。依地酸钙钠可能成为治疗急性百草枯中毒的药物,其减轻氧自由基损伤的作用值得进一步研究。

Objective： To investigate the therapeutic effect of ethylenediamine tetraacetic acid （EDTA） in the acute paraquat （PQ） intoxication and to discuss its mechanisms. Methods： 78 SD rats were divided into high, median, low therapeutic group and negative group as well as positive group randomly, all that except the negative group were given PQ at the dose of 20 mg/kg, then gave EDTA or normal saline according to the groups （therapeutic group were given EDTA 10 mg/kg, 20 mg/kg, 40 mg/kg respectively） .The pathologic changes of the lungs, and the MDA and SOD in the lung homogenate was measured.Results： The congestion and bleeding of alveolar wall were lessened after given EDTA. Compared with positive groups, the MDA decreased （P〈 0.01） and SOD increased （P〈0.01） in the lung homogenate.Conclusion： EDTA can abate the diffuse lung injury, ease the lipid peroxidation caused by oxygen free radical. It seems that it has lessened the oxygen free radical injury. EDTA may come into being the medicine used in acute PQ intoxication