河北省脊髓灰质炎实验室监测结果分析

ANALYSIS ON SURVEILLANCE OF POLIO LABORATORY IN HEBEI PROVINCE 2003-2004

目的：分析河北省脊髓灰质炎（脊灰）实验室的监测状况，及时监测可能出现的脊髓灰质炎疫苗衍生株（VDPV）或输入脊灰野病毒，以防止其扩散。方法：按世界卫生组织《脊髓灰质炎病毒检验手册》进行病毒分离与血清定型，用PCR—RFLP法和ELISA法做型内鉴定。结果：河北省脊灰实验室各项监测指标均达到世界卫生组织和卫生部的要求。2003-2004年共采集了743例急性驰缓性麻痹（AFP）病例粪例标本，所有标本用L20B、RD和Hep-23种细胞同时进行肠道病毒分离。分离到脊灰病毒（PV）67株，非脊灰肠道病毒（NPEV）74株。从AFP病例及其密切接触者粪便标本中共分离到P、79株，用血清型中和试验和PCR—RFLP法进行型内鉴定，结果为Ⅰ型8株，Ⅱ型24株，Ⅲ型20株，混合型15株，PV＋NPEV7株，疫苗变异株5株。将PV混合株进行单型分离后，所有单型毒株共107株，经ELISA试验鉴定，其中疫苗类似株（SL）104株，占97．2％，双反应株（DRV）17株，占2．8％。结论：河北省2003-2004年未发现疫苗衍生脊灰病毒或输人性脊灰野病毒。

Objective： To study the surveillance indicators in polio laboratory of Hebei, to Survell VDPV or wild - type poliovirus in time. Methods： According to WHO＇s normal method to isolated virus and neutralization test, intratypic differentiation test was carried out to all AFP isolated using PCR- RFLP and ELISA methods. Results： All the surveillance indicators in polio laboratory of hebei met the requirements of WHO and MOH. There were 743 stools which collected in 2003 - 2004. All stool .samples were also made viral isolation by, L20B cell, RD cell and Hep - 2 cell. 67 polioviruses were isolation from AFP cases. 74 of nonpolio enteroviruses （NPEV） were also isolated from AFP cases. Among 79 isolates, 8 of type I vaccine- like viyuses, 24 of type 2 vaccine- like Cruses, 20 of type 3 vaccine- like viruses, 15 of mixed viruses, 7 of vaccine- like viruses mixed with enteroviruses, 5 viruses were identified as mutant viruses. Then ELISA test was conducted, cording to WHO manual, among total 107 single serotype viruses, 104 of sabin-like viruses （SL）, 3 of double reaction viruses （DRV）. Conclusion： No wild polioviruses or VDPVs were found in Hebei. maintaining a nopolio wild- type virus status in Hebei.