几种含羟基化合物对红细胞膜流动性的影响

Influence of Hydroxylamine Derivatives and Phenolic Compounds on the Fluidity of Erythrocyte Membrane

利用氮氧自由基自旋标记物CAT-16和5-DSA较为详尽地探讨了两类含羟基化合物-羟胺衍生物与苯酚取代物对红细胞膜流动性变化的影响.实验结果表明:当非双亲性的小分子苯酚取代物插入红细胞膜内,膜的整体有序性降低和流动性增加;羟胺衍生物可作为促氧化剂诱导产生过氧化自由基,促使膜内磷脂间发生化学偶联,从而降低生物膜的内部流动性.在红细胞膜的表面,标记物分子运动基本不受偶联影响,综合效应来自小分子插入导致的膜流动性增加.

The fluidity of erythrocyte membrane in the presence of xenobiotics, such as hydroxylamine derivatives and phenolic compounds was studied by ESR in combination with spin labeling technique. The results indicated that the fluidity inside the membrane decreased evidently in the presence hydroxylamine derivatives and cumyl hydroperoxide, as results of oxidative stress and cross-link of the membrane lipids. In contrast, the mobility of the lipids on the surface of the membrane was increased, mainly due to the direct membrane effect from the small organic molecules. Accordingly, both hydroxylamine derivatives and phenolic compounds enhanced the fluidity of the surface of erythrocyte membrane.