原发性肾病综合征时血树突状细胞的变化

The variance of dendritic cell in prime nephrotic syndrome

目的:探讨树突状细胞(DC)的数量及功能在原发性肾病综合征(PNS)时的变化及意义。方法:对PNS患者14例和正常人23例进行以下方面研究,(1)DC分类试剂盒检测血DC亚群百分率;(2)分离外周血单个核细胞,细胞因子犤粒-巨噬细胞集落刺激因子(GM-CSF)、白介素4(IL-4)、肿瘤坏死因子α(TNF-α)犦体外诱导培养,流式细胞仪检测DC表型分子表达水平;(3)异体混合淋巴细胞反应(AMLR)评估DC刺激T细胞增殖的能力。结果:PNS患者外周血DC数量无明显变化,DC表达CD11c、CD40、CD80、CD83的阳性率显著低于正常人(P<0.05),表达CD1a、CD123接近正常,刺激淋巴细胞增殖的能力显著弱于正常人(P<0.01)。结论:原发性肾病综合征患者血中DC数量无明显变化,但抗原递呈能力减弱。

Objective To investigate the variance of dendritic cells （DC） from monocytes of peripheral blood in patients with prime nephrotic syndrome （PNS） . Methods studied： ① The frequency of subsets of DC was detected by Fourteen patients with PNS and 23 healthy controls were human blood DC enumeration Kit. ②The peripheral blood mononuclear cells （PBMCs） of T cell-depleted populations were incubated and induced into mature DCs in RPMI1640 medium in the presence of cytokines granulocyte-macrophage colony stimulating factor （GM-CSF）, interleukin-4 （IL-4）, tumor necrosis factor-α （TNF-α） and 100 mL/L of fetal bovine serum for a total of 9 days in vitro experiment. The expressions of surface marker on DC were detected by flow cytometry. ③ The stimulatory capacity of DC to T cell proliferation was evaluated by allogenic mixed lymphocyte reaction （AMLR） . Results The quantity of DC in patients with PNS is no more than healthy controls （P 〉 0.05）. The expression levels of CD11c, CD40, CD80 and CD83 significantly decreased, while those of CD1a, CD123 were approached to healthy controls （P 〉 0.05） . The capacity of DC to stimulate T lymphocytes decreased in PNS patients compared with healthy controls （ P 〈 0.01 ）. Conclusions The dendritic cells in patients with PNS have no variance in quantity, while have mild antigen presenting capacities.