原发性肾病综合征患者外周血树突状细胞抗原提呈功能的研究

Antigen presenting potentials of peripheral blood dendritic cells from patients with idiopathic nephrotic syndrome

目的了解原发性肾病综合征患者外周血树突状细胞的抗原提呈能力。方法采用流式细胞仪检测细胞表面标记表达,混合淋巴细胞反应评价初发原发性肾病综合征病人组(14例)和对照组(21例)外周血树突状细胞的抗原递呈能力。结果原发性肾病综合征患者外周血单核细胞能向树突状细胞分化,分化后的树突状细胞表达CD11 c、CD40、CD80和CD83的阳性率显著低于对照组(P<0.01),刺激淋巴细胞增殖的能力显著弱于对照组(P<0.05)。结论原发性肾病综合征患者外周血单核细胞向树突状细胞分化的能力减弱,分化后的树突状细胞具有较弱的抗原递呈能力。

Objective To understand the antigen presenting potentials of peripheral blood dendritic cells from patients with idiopathic nephritic syndrome. Method The phenotype of monocyte-derived dendritic cells （MoDC） was analyzed with flow cytometry and the capacity of antigen presenting of peripheral blood MoDC was evaluated by mixed lymphocyte reaction in patients with idiopathic nephrotic syndrome （ n = 14）. The data were compared with data from healthy subjects （ n = 21 ）. Results Monocytes in the peripheral blood from the patients with idiopathic nephrotic syndrome could differentiated into dendritic cells in vitro,the surface expression markers CD11c,CD40 ,CDs0 and CD83 in the patients were significantly decreased compared with that in healthy controls （ P 〈 0.01 ）. The capacity of MoDC to stimulate T lymphocytes in the patients was significantly weaker than that in healthy control （ P 〈 0.05 ）. Conclusion The monocytes possess a poor propensity to differentiate into dendritic cells in the patients with idiopathic nephrotic syndrome,and these differentiating dendritic cells have mild antigen presenting ability.