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端粒酶在年龄相关性白内障晶状体上皮细胞中的表达及意义 被引量：3

Expression and significance of the telomerase activity in human age-related cataract lens epithelial cells
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摘要 目的探讨年龄相关性白内障及正常晶状体上皮细胞的端粒酶活性的表达及其在年龄相关性白内障发生发展中的作用。方法采用TRAP-银染法及TRAP-ELISA两种方法检测9例年龄相关性白内障、3例正常晶状体上皮细胞的端粒酶活性。结果9例年龄相关性白内障及3例正常晶状体上皮细胞中端粒酶均呈阳性,年龄相关性白内障组晶状体上皮细胞中端粒酶活性明显低于正常组,经统计学分析差别具有显著意义(P<0.05)。结论端粒酶活性的降低可能是年龄相关性白内障晶状体上皮细胞发生凋亡的一个重要机制,端粒酶在年龄相关性白内障的发生发展中起着重要的作用。 Objective To investigate the telomerase activity in human lens epithelial cells of age-related cataract and normal lens and evaluate the role of telomerase in the occurrence and progression of age-related cataract. Methods TRAP-sliver staining method and TRAP-ELISA method were used to detect the telomerase activity in lens epithelial cells from 9 agerelated cataracts and 3 normal lens from human, Results Telomerase activity was detected in all specimens. Compared to normal lens epithelial cells,telomerase activity in lens epithelial cells from age-related cataract was decreased, The difference was significant between two groups （0. 5371±0. 3407 vs 1.2077±0. 1545, P 〈 0. 05 ）. Conclusion The decrease of telomerase activity may be an important mechanism of apoptosis of lens epithelial cells. Telomerase play an important role in the occurrence and progression of age-related cataract.
作者 徐国兴 武莉莉 郑学栋 胡建章 王婷婷 林鸿
机构地区 福建医科大学第一附属医院福建省眼科研究所
出处 《眼科研究》 CAS CSCD 北大核心 2005年第6期593-595,共3页 Chinese Ophthalmic Research
基金 福建省自然科学研究基金资助(2001Z033)
关键词 年龄相关性白内障 端粒酶 TRAP-银染法 TRAP-ELISA age-related cataract telomerase TRAP-sliver staining method TRAP-ELISA method
分类号 R776.1 [医药卫生—眼科]
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参考文献13

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共引文献40

同被引文献35

  • 1张楠,郑广瑛.端粒酶与年龄相关性白内障的关系[J].眼科新进展,2005,25(2):184-186. 被引量：4
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眼科研究
2005年 第6期

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