知母皂甙对Aβ_(25-35)激活的小鼠腹腔巨噬细胞TNF-α和iNOS表达的抑制作用

Inhibitory Effect of Saponins from Anemarrhena Asphodeloides Bge on Expression of Aβ_(25-35)-Induced TNF-α andiNOS in Peritoneal Macrophages Culture

目的观察知母皂甙(SAaB)对Aβ25-35激活的小鼠腹腔巨噬细胞TNF-α和iNOS表达的抑制作用,并初步探讨其作用机制。方法分离小鼠腹腔巨噬细胞进行培养,用10μg/L的Aβ25-35刺激细胞,对照组同时加入不同浓度(10μg/L、30μg/L、100μg/L)的SAaB共同孵育。采用ELISA法、Griess反应和免疫组织化学(SP)方法分别检测TNF-α分泌量、iNOS表达、NO的生成量。结果Aβ25-35能激活小鼠腹腔巨噬细胞,使其分泌的TNF-α增多、iNOS表达上调、NO的生成量增加。SAaB能有效地抑制此变化,并呈剂量依赖关系。结论这一作用的机制可能与其抑制激活的巨噬细胞释放炎症因子有关。

Objective To investigate inhibitory effect of Saponins from Anemarrhena asphodeloides Bge on expression of Aβ25-35 induced TNF-α and iNOS in peritoneal macrophages culture and to explore the relative mechanisms of the protection. Methods The isolated peritoneal macrophages from mice were cultured. The cultured macrophages were exposed to Aβ25-35 （10μmol/L） to stimulate cell, whereas the ant-stimulate groups were added with SAaB of various concentrations （10μg/L, 30μg/L, 100μg/L） . Production of TNF-α was detected by ELISA method. The expression of iNOS protein was detected by immunhistochemieal technique and production of NO was determined with Griess reaction technique. Results Aβ25-35 could activate peritoneal macrophages of mice. Aβ25-35 treatment resulted in the increase of TNF-α, NO production, and iNOS expression. SAaB significantly suppressed these change in a dose-dependent fashion. Conclusions SAaB could inhibit Aβ-induced inflammatory response in macrophage culture.