大鼠盲襻综合征模型的建立

A rat model of blind loop syndrome

目的:建立一种费时短的大鼠盲襻综合征(BLS)模型。方法:35只SD大鼠随机分为两组:对照组7只,不行任何干预;实验组28只,行空肠造襻手术。检测体重、肠液菌落数、血常规和血生化。结果:实验组术后7天盲襻细菌菌落数>105个/m l,14天体重增长停滞,与对照组比较,实验组术后28天出现血红蛋白下降,为(144.8±9.4)g/Lvs(155.0±5.1)g/L,P<0.05;平均红细胞体积增加,为(77.9±5.7)flvs(57.6±1.6)fl,P<0.01;血清总蛋白下降,为(42.9±7.3)g/Lvs(60.7±7.6)g/L,P<0.01;清蛋白下降,为(26.8±4.4)g/Lvs(35.6±2.5)g/L,P<0.05。血钙、钠、钾、氯离子浓度无显著性改变。结论:该模型是一种成功的大鼠BLS模型。

Objective： To establish a rat model of blind loop syndrome. randomized into 2 groups. Control group had no operation. Blind loop group self-filling blind loops created at the distal end 5 cm away from the ligament and compared with control animals. Results： Blind loop bacterial overgrowth Methods： SD rats were animals, suffered 15 cm of Treitz, were observed took place in seven days （ 〉105 organisms/ml）. Two weeks after the operation, all blind loop rats had no weight-gaining. Four weeks after the operation, all blind loop rats had greater mean corpuscular volume （77.9±5.7 ） fl vs （57.6 ±1.6） fl （ P〈0.01 ）, lower levels of hemoglobin （ 144.8±9.4 ） g/L vs （ 155.0±5.1 ） g/L （ P 〈 0.05 ）, serum total protein （ 42.9±7.3 ） g/L vs （ 60.7±7.6 ） g/L （ P〈0.01 ） , and albumin （ 26.8±4.4 ） g/L vs （ 35.6±2.5 ） g/L （ P〈0.05 ）, than the control group animals. Conclusion ： This modified model is a more feasible rat model of blind loop syndrome.