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水稻中一个25 kD Bowman-Birk蛋白酶抑制剂的表达和抑制活性分析 被引量：6

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摘要 水稻Bowman-Birk蛋白酶抑制剂(RBBI)在体外是胰蛋白酶抑制剂,有的有1个同源结构域(8kD),有的有2个同源结构域(16kD).本研究发现,在水稻体内存在带有3个同源结构域的RBBI(25kD),而且RBBI受发育和伤害调控.体外实验发现,3:13二硫键(而不是4:5二硫键)可抑制胰蛋白酶的活性;而RBBI3-1的D3结构域对胰蛋白酶、胰凝乳蛋白酶、木瓜蛋白酶和枯草芽孢杆菌素没有抑制活性.突变实验表明,改变D1结构域N端的P1位点(把Lys突变为Leu)并不能获得对胰凝乳蛋白酶的抑制活性,这表明RBBI3-1的D1结构域反应回环阻碍了P1位点获得新的抑制活性;而对胰凝乳蛋白酶的抑制活性可能还需要其他结构(比如3:13二硫键)的参与.
作者 陈军 毛盛勣 谢阳 曹忠仁 张彦 刘静 陈章良 瞿礼嘉 顾红雅
机构地区 北大-耶鲁植物分子遗传及农业生物技术联合研究中心 国家植物基因研究中心(北京)
出处 《科学通报》 EI CAS CSCD 北大核心 2005年第22期2501-2508,共8页 Chinese Science Bulletin
基金 国家重点基础发展规划(批准号:G2000016200,G1999011602) 国家高技术研究发展计划(批准号:2002AA2Z1001-07) 洛氏基金(批准号:GN97003)资助项目.
关键词 Bowman-Birk抑制剂 胰蛋白酶抑制活性 胰凝乳蛋白酶抑制活性 二硫键
分类号 S511 [农业科学—作物学]
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参考文献28

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  • 2Tashiro M, Hashino K, Shinozaki M, et al. The complete amino acid sequence of rice bran trypsin inhibitor. J Biochem, 1987, 102: 297～306.
  • 3Prakash B, Selvaraj S, Murthy M R N, et al. Analysis of the amino acid sequences of plant Bowman-Birk inhibitors. J Mol Evol, 1996, 42: 560～569.
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二级参考文献27

  • 1谢明,陈新,瞿礼嘉,刘虹,顾红雅,陈章良.一种水稻蛋白酶抑制剂基因的克隆及其结构分析[J].Acta Botanica Sinica,1996,38(6):444-450. 被引量：5
  • 2Ryan C A. Proteinase inhibitors, a comprehensive treatise. In: Stumpf P K, Conn E E, eds. The Biochemistry of Plants, vol 6. New York: Academic Press, 1981. 351～371
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  • 6Qu L J, Chen J, Liu M H, et al. Molecular cloning and functional analysis of a novel type of Bowman-Birk inhibitor gene family in rice. Plant Physiol, 2003, 133: 560～570
  • 7Odani S, Koide T, Ono T. Wheat germ trypsin inhibitors. Isolation and structural characterization of single-headed and double- headed inhibitors of the Bowman-Birk type. J Biochem, 1986, 100: 975～983
  • 8Tashiro M, Asao T, Hirata C, et al. The complete amino acid sequence of a major trypsin inhibitor from seeds of foxtail millet(Setaria italica). J Biochem(Tokyo), 1990, 108: 669～672
  • 9Mello M O, Tanaka A S, Silva-Filho M C. Molecular evolution of Bowman-Birk type proteinase inhibitors in flowering plants. Mol Phylogene Evol, 2003, 27: 103～112
  • 10Tashiro M, Hashino K, Shiozaki M, et al. The complete amino acid sequence of rice bran trypsin inhibitor. J Biochem, 1987, 102: 297～306

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科学通报
2005年 第22期

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