以豆粕为原料制备反刍动物饲用肽的研究

The preparation technology of soybean-peptide from soybean meal for ruminant

本文通过正交试验,选用碱性微生物蛋白酶,研究得出酶解法制备大豆肽的最佳工艺参数:豆粕预处理条件为90℃水浴加热10min,酶解条件为底物浓度5%(W/V)、加酶量5万单位/g蛋白质、温度50℃、pH值10、酶解时间5.5h。蛋白质水解率达到25%,平均肽链长度为4.0。制得大豆肽粗蛋白质含量66.83%(DM)。并对制得大豆肽和原料豆粕的氨基酸含量进行分析。

An orthogonal test experiment was conducted on the preparation of soybean-peptide from hydrolyzing soybean meal.Alcalase 2709 was selected to hydrolyse soybean meal, and the proteolytic characteristics were investigated. The protein was pretreated in 90℃ water bath for 10 min.The optimum conditions for enzymatic hydrolysis consisted of substrate concentration=5 % （W/V）, enzyme concentration=5 units/gram substrate protein, temperature=50℃, pH= 10, reaction time=5.5 h.Degree of hydrolysis（DH）could reach to 25 % and average peptide link length（APLL）was 4.0.The crude protein concentration of the peptide powder was 66.83 %（based on DM）.Amino acids analyses of peptide products and soybean meal stocks were also conducted.