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Genistein影响人催乳素瘤细胞增殖及机制

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摘要 目的研究genistein对人催乳素瘤细胞的增殖代谢和DNA合成的影响,并深入探讨genistein影响催乳素瘤细胞增殖作用的机制,观察genistein作用后催乳素瘤细胞内蛋白激酶C(PKC)及cAMP/cGMP的变化。方法采用MMT和[3H]TdR掺入实验检测genistein对人催乳素瘤细胞增殖和DNA合成的影响;通过PKC活性及cAMP和cGMP含量测定,深入探讨genistein影响催乳素瘤细胞增殖分化的分子机制。结果①Genistein抑制催乳素瘤细胞的增殖和DNA合成,并呈剂量依赖性;②与空白处理组相比,使用PKC的激动剂PMA处理培养的人催乳素瘤细胞时可使胞膜和细胞总PKC活性浓度均升高,但genistein(10μmol/L)作用15min后,胞浆、胞膜和细胞总PKC活性均下降;③Genistein作用于人催乳素瘤细胞15min后,胞内cAMP水平显著升高,而cGMP没有明显改变。结论实验结果为探讨genistein抑制催乳素瘤细胞增殖的分子机制提供了重要线索,同时提示,genistein对催乳素瘤细胞增殖分化的调控作用是细胞内多信息系统相互整合的结果。
机构地区 成都军区总医院
出处 《四川医学》 CAS 2005年第12期1402-1404,共3页 Sichuan Medical Journal
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