原发性肝癌患者血清TGFβ1和IL-8水平与预后的关系

Study on the Relationships between Serum TGFβ1, IL-8 and Prognosis in Primary Hepatocellular Carcinoma

目的:探讨原发性肝癌(肝癌)患者血清转化生长因子β1(TGFβ1)、白细胞介素-8(IL-8)、T细胞亚群水平及其与预后关系。方法:应用ELISA、放射免疫法及流式细胞术检测24例肝癌患者(HCC组)血清TGFβ1、IL-8及T细胞亚群水平,并与25例健康献血者(NC组)和25例乙型肝炎肝硬化者(LC组)比较。结果:HCC组血清TGFβ1、IL-8水平较NC组和LC组明显升高,HCC组和LC组CD4+细胞和CD4+/CD8+比值较NC组明显降低,CD8+细胞明显升高;HCC组血清TGFβ1水平与CD4+细胞数量及与CD4+/CD8+比值呈负相关,与CD8+细胞呈正相关。血清TGFβ1、IL-8水平及CD4+/CD8+比值与肝癌分期有关。结论:观察血清TGFβ1、IL-8及T细胞亚群水平的改变有助于肝癌的诊断及对预后的评价。

Objective： To investigate the serum levels of TGFβ1,IL-8 and T cell subset and their relationships with prognosis in hepatocellular carcinoma （HCC）. Methods： TGFβ1, IL-8 and T cell subsets were detected by ELISA, radioimmunoassay and three-color flow cytometry in 24 HCC patients and compared with those in normal controls （NC） and liver cirrhosis （LC） patients. Results： The Serum levels of TGFβ1, and IL-8 were significantly elevated in patients with HCC compared with LC and NC. compared with NC, CD4^＋ T cell and CD4^＋/CD8^＋ ratio decreased and CD8 ^＋ T cells increased in patients with HCC. There was a negative correlation between TGFβ1 level and CD4^＋ T cell number, and CD4^＋/CD8^＋ ratio, and a positive correlation between TGFβ1 level and CD8^＋ T cells number. The levels of TGFβ1, IL-8 and CD4^＋/CD8^＋ ratio correlated with advanced tumor node stage. Conclusion： The levels of TGFβ1, IL-8 and T cell subset are useful index in diagnoses and estimate the prognosis in HCC.