康宁木霉固态发酵秸秆生产纤维素酶的研究

Studies on Solid State Fermentation for Cron Stalk by Trichoderma koningii Producing Cellulase

采用康宁木霉(Trichodermakoningii)固体发酵生产纤维素酶,研究了秸杆粉和麦麸用量、料水比、起始pH值、温度和时间对该菌株产纤维素酶活力的影响。结果表明,康宁木霉的适宜发酵条件为:秸秆∶麦麸=3∶2,料水比1∶2,培养温度28～30℃,起始pH5.5～6.0时产酶活力最高。在适宜培养条件下,发酵周期为72h,发酵液中FPA酶活为172.3μmol/h.mL。

Cellulase production was carried out by solid state fermentation with Trichoderma koningii. The effects of wheat bran and cron stalk ratio, water content ratio, initial pH of medium, temperature, time on cellulase synthesis were studied. The results show that the optimum conditions of producing cellulase are： cron stalk and wheat bran ratio is 3 ： 2, water contents ratios 1 ： 2, the temperature is 28-30℃, the initial pH is 5.5-6.0. Under the above conditions and growing 72 h, the activity of FPase in the fermented is 172.3 μmol/h·mL.