摘要
为探讨小鼠乳腺癌实体瘤(Ca761/S)的细胞遗传学特性,我们进行了染色体G、C带核型分析。含46条的占56%,含40条的占44%,共发现6条标记染色体,出现频率为10/10。这种稳定出现的超二倍体和假二倍体核型及恒定出现的标记染色体,提示肿瘤细胞恶性变过程中占有生长优势的细胞经体外接种、悬浮培养、分离、再培养有可能克隆化。此外,为了进一步搞清标记染色体的来源,我们对C带技术做了某些改进。
The cytogenetical feature of murine solid mammary carcinoma (Ca761/s) was analysed with G-banding and C-banding technique.The results show that the chromosome number 46 takes 56%,and number 40 takes 44% in Ca76/s model.There are six marker chromosomes and the frequency in 10/10.The stable appearance of superdeploid and pseudodeploid karyotypesand the marker chromosomes suggest that the (Ca76/s) cell model should be cloned.We also did some improvement in C-banding technique.
出处
《大连医科大学学报》
CAS
1996年第2期133-135,共3页
Journal of Dalian Medical University
关键词
核型
G带
C带
标记染色体
乳腺癌
Karyotype
G-banding C-banding
marker chromosome