转Bt/CpTI基因棉花抗虫性鉴定与筛选

Identification and Screening on Insect Resistance of Bt/CpTI Transgenic Cottons

以国内育成和国外引进的52个转Bt/CpTI基因棉花品种(系)为材料,在河北农业大学棉花育种圃种植,采用室内生物鉴定方法,研究供试转基因棉花的棉铃虫抗性和甜菜夜蛾抗性,并进行抗虫性筛选。结果表明:国内育成的品种(系)在棉田二代棉铃虫危害期对棉铃虫的抗性不低于国外引进的品种(系);在三代棉铃虫危害期对棉铃虫的抗性,国内育成的品种(系)还略高于国外引进品种。新棉33B对甜菜夜蛾表现高抗,其他品种抗性一般。转Bt和CpTI的双价抗虫棉对甜菜夜蛾幼虫的抗性不高。根据供试品种(系)的抗虫性鉴定结果,筛选出SGK321等15个在二代、三代棉铃虫危害期对棉铃虫表现高抗的品种(系)和兼抗棉铃虫、甜菜夜蛾的新棉33B。

Fifty-two Bt/CpTI transgenic cottons from home and abroad have been used to study insect resistance to Helicoverpa armigera and Laphygma exigua. The tested varieties were planted in the diseases-free experimental field and insect-resistance was determined in the laboratory, It was showed that the resistance of China-bred varieties to the second-generation H, armigera was lower than that of the varieties introduced from abroad, As to the third-generation H, armigera, China-bred varieties were somewhat higher than the overseas varieties. Xinmian 33B had higher resistance to L. exigua, The Bt and CpTI transgenic varieties showed low resistance to L. exigua. According to identification of insect-resistance, 15 varieties were screened out with higher resistance to the second and third-generation H. armigera and Xinmian33B with higher resistance to both H, armigera and L. exigua.