角质细胞生长因子受体腺病毒表达载体在脂多糖所致肺泡上皮细胞损伤修复中的应用

Application of adenovirus expression vector of KGFR in repairing LPS-induced alveolar epithelial damage repair

目的观察KGFR腺病毒表达载体在脂多糖(LPS)致A549细胞损伤模型中转基因表达KGFR的情况及其促使A549细胞对抗LPS损伤的效果,为基因治疗急性肺损伤探索有效途径。方法KGFR腺病毒表达载体感染LPS致伤前后的A549细胞,通过形态学观察转基因作用对LPS致伤前后A549细胞的影响,并运用western blot检测KGFR腺病毒表达载体在LPS致伤前后的A549细胞中转基因表达KGFR蛋白情况。结果KGFR腺病毒表达载体感染LPS致伤前后的A549细胞,转基因组对抗LPS致伤作用明显,western blot检测表明KGFR腺病毒表达载体转基因表达KGFR蛋白效果显著(P<0.05)。结论KGFR腺病毒表达载体能够转基因表达KGFR蛋白,并具备转基因治疗急性肺损伤的能力。

Objective To find an effective treatment of acute lung injury （ALI） with gene therapy, an adenovirus expression vector KGFR had been utilized for observing the state of transgene KGFR in A549 cell line, and the effect of the vector in facilitating A549 cell line to anti-LPS-induced damage. Methods Both LPS-injured and normal A549 cell lines had been transfacted by adenovirus expression vector KGFR. The effect of transfaction and the expression of KGFR after transfaction was measured by morphology and western blot respectively. Result There is a significant effect of anti-LPS- induced damage of A549 cell line after transfaction with adenovirus expression vector KGFR. Detection of western blot indicated that the adenovirus expression vector KGFR had significant effect on transgene and KGFR protein expression （ P 〈 0.05 ）. Conclusion The adenovirus expression vector KGFR could express KGFR protein after transgene experiment. It could be a competent vector to cure ALl by gene therapy.