普乐可复和/或骁悉联合阿苯哒唑脂质体治疗感染泡球蚴大鼠心脏移植的疗效观察

Observation of the effect of combined adminisitration of FK506, MMF and L-ABZ on SD-to-Wistar rat cardiac graft survival

目的:探讨免疫抑制剂普乐可复(FK506)和/或骁悉(MMF)联合抗包虫病药阿苯哒唑脂质体(L-ABZ)治疗泡球蚴感染大鼠心脏移植的疗效。方法:建立SD大鼠对Wistar大鼠心脏移植模型。泡球蚴感染的52只Wistar 雄性大鼠随机分为6组,分别单独或联合给予FK506、MMF、L-ABZ进行治疗,判断心脏排斥后,观察移植物存活时间。结果:FK506+MMF治疗组、感染对照组大鼠移植心脏存活时间分别为(16.11±8.04)d、(11.6±6.02)d,2组相比差异有统计学意义(P<0.05),其余各组大鼠移植心脏存活时间与感染对照组相比未见明显延长(P>0.05)。结论:(1)单独应用L-ABZ不会影响移植物的存活;(2)L-ABZ与FK506+MMF可能有一定的拮抗作用。

Objective： To study the effect of combined adminisitration of tacrolimus （FK506）, mycophenolic acid （MMF）, and L-ABZ on SD-to-Wistar rat cardiac graft survival. Methods： Fifty-two healthy SD rats and 52 E.m. infected Wistar rats were used in the study. The animals were randomly divided into： Groupl SD-to-Wistar（n = 8）, without any treatment; Group 2 SD-to-Wistar（n = 9）, L-ABZ 50 mg·kg^-1·d^-1 Group 3 SD-to-Wistar（n = 8）, FK506 1 mg·kg^-1·d^-1; Group 4 SD-to-Wistar（n = 9）, FK506 1 mg·kg^-1·d^-1、L-ABZ 50 mg·kg^-1·d^-1; Group 5SD-to-Wistar（n = 9）, FK506 1 mg·kg^-1·d^-1、MMF20 mg·kg^-1·d-1; Group6 SD-to-Wistar（n = 9）, FK506 1 mg·kg^-1·d^-1、MMF20 mg·kg^-1·d^-1、L-ABZ 50 mg·kg^-1·d^-1. All the agents were administrated orally one time per day. Graft samples were harvested for histopathological examination and immunohistochemical study. Results： FK506＋MMF can significantly prolong the survival time of graft vs control （16.11±8.04 vs 11.6±6.02, P〈0.05）. The survival time of the grafts in all other groups were not significantly prolonged. Conclution： （1） L-ABZ could not affect the survival of graft alone. （2） Use of L-ABZ might antagonize the effect of the FK506＋MMF.