涤痰汤对大鼠脑缺血再灌注损伤神经细胞凋亡的影响

The Influence of Ditan Decoction on Apoptosis of Nervous Cell by Cerebral Ischemia/reperfusion Injury in Rats

目的 探讨涤痰汤对大鼠局灶性脑缺血再灌注损伤细胞凋亡的影响。方法 清洁级成年Wistar雄性大鼠21 只,体重250-300g,随机分成假手术组、模型组、涤痰汤组。采用改良线栓法制成局灶性脑缺血再灌注损伤模型。于大鼠 苏醒后,假手术组及模型组予生理盐水灌胃,治疗组予涤痰汤灌胃。术后72h,对以上各组大鼠行神经功能缺损程度评分和 细胞凋亡率检测。结果 与模型组比较,涤痰汤组神经功能缺损程度显著改善(P<0．05),缺血侧脑组织细胞凋亡率明显 降低(P<0．05)。结论 涤痰汤可改善脑缺血再灌注损伤大鼠的神经功能缺损程度,可能通过降低脑组织细胞凋亡率发挥 神经保护作用。

To investigate the influence of Ditan decoction on apoptosis of cerebral ischemic-reperfusion injury in rats. Methods： Twenty-one of male Wistar rats weighting 250-300g were randomly divided into three groups： shamoperated group, control group and Ditan decoction group. The focal cerebal ischemie-reperfusion model was established with thread embolism of middle cerebral artery. After suffering 2h occlusion and following 70h reperfusion, we measure the neurologic function score and apoptosis. Results： Compared with the control group, in Ditan decoction group, the neurologic function score was remarkly falling （P〈 0.05）, the neural apoptosis clearly cut down （P〈 0. 05）. Conclusion： By the possible mechanism of restraining apoptosis, Ditan decoction has protective effect on focal cerebral ischemie-reperfusion injury in rats.