异种脱蛋白松质骨载体的制备及性能评价

Experimental study on properties of xenogeneic deproteinised cancellous bone as scaffold of bone tissue engineering

目的研究探讨异种脱蛋白松质骨性能,以制备综合性能优异的骨组织工程载体材料。方法我们将取材于成年猪股骨远端松质骨通过理化方法制作成脱蛋白松质骨载体,并对载体形态结构、组成成分、载体与种子细胞黏附及生长增殖情况以及生物力学特性进行检测分析。同时将载体材料复合自体骨髓间充质干细胞和骨形态生长因子植入成年山羊横突间进行组织工程化成骨融合,观察成骨情况,并通过免疫酶组织染色方法检测异种脱蛋白松质骨的免疫原性。结果脱蛋白松质骨具有天然网状孔隙结构,其无机成分为羟基磷灰石,有机成分为Ⅰ型胶原,力学性能保存良好,有良好的细胞相容性,免疫原性检测阴性。异种脱蛋白松质骨复合rhBMP-2和MSCs植入体内后多点成骨,效果满意。结论异种脱蛋白松质骨是一种良好的载体材料。

Objective To study the properties of xenogeneic deproteinised cancellous bone and to prepare scaffold in bone tissue engineering. Methods We made deproteinized bone derived from pigs and goals femoral cancellous bone by physical and chemical treatments. Their morphological features, constitute components and mechanical properties were examined by scanning electronmicroscopy,x-rays diffraction analysis, mechanical experimental instrument and so on, Its cellular compatibility was examined by MSCs culture in vitro. Then the scaffolds composited with self mesenchymal stem cells（MSCs） were implanted at intertransverse site of goals. To study the osteogenesis possibility of xenogeneic and heterologous deproteinised bone. Immune response was studied through gross observation. Results It showed that deproteinised cancellous bone maintained natural network pore system. The main organic material is collagen Ⅰ and inorganic is hydroxyapatite. It hardly has any antigen and it has good histocompatibility. Deproteinised cancellous bone composited with MSCs, rhBMP-2 could form cartilage and hone in vivo and more new bone tissue formed gradually as time went by, Conclusion The osteogenesis of deproteinised cancellous bone composited with MSCs in vivo is satisfied, It could be used as scaffold in bone tissue engineering,