GABA_Aα_1受体在局灶性皮质发育障碍大鼠脑皮质中的表达

Expression of GABA_Aα_1 receptor in experimentally induced disorders of cortical development

目的探讨液氮损伤诱导局灶性皮质发育障碍大鼠脑皮质中γ-氨基丁酸A亚型α1(GABAAα1)的表达。方法实验随机分为液氮损伤组、假手术组和正常对照组,先建立局灶性皮质发育障碍模型,采用常规HE和Nissl染色,免疫组织化学方法用SABC法,DAB显色。观察微脑回和周围组织的结构及其GABAAα1受体阳性细胞表达情况。结果在大鼠脑嘴尾方向形成了一小的脑回,小脑回及前额叶GABAAα1受体阳性着色神经元在损伤组中的表达减少(q检验P<0.05)。结论微脑回及周围结构里GABAAα1受体数量的下调可能是其导致癫痫发作的原因之一。

Objective To use a rat model of focal cortical neuronal migration disorders（NMD）（microgyria） for determining the expression of GABAAα1 receptor in liquid nitrogen lesions induced disorders of cortical development. Methods Newborn Wistar rats （〈24 hr old;n= 12） were cooled with liquid nitrogen on the exposed calvaium above the primary parietal cortex （parl）. Freeze lesioned and sham operated or normal rats were allowed to survive for 16- 18 weeks before histochemical and immunohistochemical studies. We observed the structure of microgyrus and expression of GABAAα1 receptor in the animal model. Results All freeze lesioned animals displayed typical cortical malformations consisting of a longitudinal microgyrus. There are chances of GABAAα1 receptor in cortical dyspiasia. Conclusion Expression of GABAAα1 receptor in adjacent to the experimental microgyrus suggest that an imbalance of inhibition and excitation could be one of mechanisms in an intrinsic epileptogenicity of cortical dysplasias,it is important to result in epileptogenesis as to the decrease in GABAAα1 receptor density and the functional impairment in GABAergic inhibition.