34例婴幼儿呼吸道合胞病毒肺炎T辅助淋巴细胞亚群的改变

Change of T assistant lymphocyte subsets of 34 infants with respiratory syncytial virus pneumonia

目的探索呼吸道合胞病毒(RSV)肺炎患儿细胞免疫状况的变化。方法采用流式细胞仪检测RSV肺炎急性期患儿及正常对照组外周血T辅助淋巴细胞1(Th1)和T辅助淋巴细胞2(Th2)的百分率、CD4+细胞百分率、CD3、CD8、NK细胞、B细胞百分率及CD4/CD8比值。结果RSV肺炎急性期患儿的外周血Th1、Th2的细胞百分率〔(11.96±6.26)%、(3.45±2.03)%〕均显著高于对照组〔(9.16±9.90)%、(2.14±1.12)%〕,(P<0.05);但RSV肺炎组Th1/Th2之比值、CD3、CD8、NK细胞、B细胞百分率及CD4/CD8比值,均与正常对照组之间无统计学差异(P>0.05)。结论RSV感染可活化T辅助淋巴细胞亚群,表现为Th1和Th2细胞百分率均增加,Th1和Th2细胞免疫反应增强。

Objective To investigate the alteration of cellular immunity in the respiratory syncytial virus （RSV） pneumonia. Methods Detecting the expression of T assistant lymphocyte subsets in peripheral blood of young children with RSV pneumonia in comparison with normal children using flow cytometer, including the percentage of Thl, Th2, CD4^＋ cells, NK cell, B cell, CD3, CD8 cells, and the ratio of CD4/CD8. Results The percentage of Thl and Th2 [（11.96 ± 6.26） % ,（3.45 ± 2.03）% ] were significantly greater compared to normal children [（9.16±9.90）%, （2.14±1.12）%] ,P〈0.05. There was no difference between RSV pneumonia comparison and normal children in the ratio of Th1/Th2,CD3,CD8 cells, percentage of Nk cell, B cell and the ratio of CD4/ CD8. （P〉0.05）. Conclusion T assistant lymphocyte subsets can be activated by RSV infection, displaying the increase of the percentage of Th1 and Th2. The cellular immunity reaction of Th1 and Th2 enhances.