职业性接触抗肿瘤药物甲氨喋呤工人的遗传损伤

Investigating genetic damage of workers occupationally exposed to methotrexate

目的用3个遗传终点来研究接触甲氨喋呤(MTX)工人的遗传损伤。方法外周血分别来自21名生产MTX工人和21名对照组工人。用微核试验、彗星试验、hprt基因突变试验和TCR基因突变试验对两组人群进行检测。结果工人组平均微核率(MNR)、平均微核细胞率(MCR)分别为10.10‰±0.95‰和8.05‰±0.75‰,明显高于对照组(5.48‰±0.82‰和4.38‰±0.58‰),差异有统计学意义(P<0.01)。工人组和对照组的平均尾长(MTL)分别为(1.30±0.06)、(0.07±0.01)μm,差异有统计学意义(P<0.01)。但两组平均尾相(MTM)的差异无统计学意义(P>0.05)。工人组的hprt和TCR平均突变率分别为1.00‰±0.02‰和(6.87±0.52)×10-4,明显高于对照组[0.86‰±0.01‰和(1.67±0.14)×10-4],差异有统计学意义(P<0.01)。结论生产MTX工人某种程度上存在遗传损伤。

Objective To study genetic damage of workers alone occupationally exposed to methotrexate （MTX） with three end-points. Methods The blood samples from 21 workers exposed to MTX and 21 controls were detected with micronueleus test, comet assay, hprt gene mutation test and TCR gene mutation test. Results Themean mieronuclei rate（MNR） and mean mieronueleated cells rate（MCR） in 21 workers were 10.10‰ ± 0.95‰ and 8.05‰ ± 0. 75‰ , respectively, which were significantly higher than those （5.48‰ ± 0.82‰ and 4.38‰ ±0.58 ‰ ） in eontrol（P 〈 0.01） .The mean tail length（MTL） of 21 workers and 21 controls were （ 1.30 ± 0.06） μm and （0.07 ± 0.01 ） μm, respectively, there was significant difference between workers and controls （P 〈 0.01 ）. But the difference between workers and controls for mean tail moment（MTM） was not signifieant（P 〉 0.05）.The average mutation frequeney（Mf-hprt） of hprt and （Mf-TCR） of TCR in workers were 1.00‰ ± 0.02‰ and （6.87 ± 0.52） × 10^-4,respectively,which were significantly higher than thoseE0.86‰ ± 0.01‰ and （1.67 ± 0.14） × 10^-4] in eontrol（P 〈 0.01）. Conclusion The genetic damage to some extent appeared in workers occupationally exposed to methotrexate.