子宫内膜异位症患者腹腔液中细胞因子RANTES的测定

Measurement of cytokine RANTES in peritoneal fluid of patients with endometriosis

目的:探讨细胞因子RANTES在子宫内膜异位症(EM)发病中的作用。方法:应用酶联免疫吸附试验对12例非EM(对照组),12例Ⅰ,Ⅱ期EM(轻度组)及16例Ⅲ,Ⅳ期EM(中重度组)患者腹腔液中RANTES水平进行测定。对RANTES水平与EM评分(RAS)及痛经评分进行相关性分析。结果:中重度组腹腔液中RANTES水平明显高于对照组(P<0.01)。轻度组与中、重度组之间及对照组与轻度组之间均有显著性差异(P<0.05)。RANTES的水平与RAS评分存在显著相关性(P<0.01),与痛经评分无相关性(P>0.05)。结论:EM患者腹腔液中RANTES水平异常升高在该病的发生发展中起重要作用。

Objective： To measure the changes of eytokine RANTES concentrations in peritoneal fluid of patients with endometriosis, and to explore the role of this cytokine in the pathogenesis of endometriosis. Methods： The RANTES concentrations in peritoneal fluid from 12 normal control subjects, 12 patients with mild endometriosis（ stage Ⅰ and Ⅱ ）, and 16 patients with moderate to severe endometriosis （stage Ⅲ and Ⅳ） were measured by using enzyme-linked immunosorbent assay （ELISA）. The correlation analysis between RANTES concentrations in peritoneal fluid and the severity of endometriosis or dysmenorrhea score were performed. Results： RANTES concentrations in peritoneal fluid of patients with endometriosis were significantly higher than those in controls（ P 〈0.01）. There was significant difference in RANTES concentrations between patients with mild endometriosis and those with moderate to severe endometriosis（ P 〈0.05）. The RANTES concentrations were significantly correlated with the severity of endometriosis（ P 〈 0.01 ）, but no significant correlation was found between RANTES concentrations and dysmenorrheal score （ P 〉 0, 05）. Conclusion： The abnormally elevated RANTES concentrations in peritoneal fluid may play an important role in the pathogenesis of endometriosis.