摘要
于1990年3月用β-琼胶酶对多管藻的冷水提取多糖进行酶解,酶解液通过DEAE-SephadexA25和Bio-GelP6色谱柱分离,分离出2个带电荷的寡糖A—Ⅰ和A—Ⅱ.经1H-NMR和13C-NMR光谱法分析,确定结构式分别为61,22-二-O-甲基-63-硫酸基-新琼四糖和61,22-二-O-甲基-63,63-二硫酸基-新琼六糖,证明它们构成多管藻多糖分子的单位组分。
The polysaccharide extracted by cold water from PolyslPhonta urceolata collected on March 1990, in Qingdao, was hydrolyzed using β-agarase fromPseudomonas atlantica. The mixture after hydrolysis was precipitated by adding 95%ethanol solution, tYren the uPper clear solution was evaporated in vacuo. this ethanolconeentrate of enzyndc hydrolyzate was chromatographed on DEAE-Sephadex A25and then Bio-Gel P6 columns. Two charged agarooligomers A-|Ⅰ and A -Ⅱ were iso-lated from the eluents. Their degrees of polymerization were estimated by 1H-NMRspectra to be 2 and 3, respectively. Two oligomers gave clear resonance signals at3.41ppm and 3.52ppm in 1H-NMR, which showed the presence of methyl groups onC2 of L-galactose and C6 of D-galactose, respectivcly. A strong signa at 4.18ppm indicated that a sulfate group links with C6 of D-galactose. The structure of theoligomers A-Ⅰ and A-Ⅱ was deduced by the assignment of chendcal shifts in 1H-and 13C-NMR spectra to be 61, 22-di -nethyl -meooprotetraose -63 -sulfate and61, 22-di -methyl -neoagarohexaose - 63, 65 -disulfate, respectively, which constitutethe unit component of the polysaccharide of Polysiphonia urceolata.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
1996年第5期505-510,共6页
Oceanologia Et Limnologia Sinica
基金
国家自然科学基金!3870137
关键词
Β-琼胶酶
多管藻
多糖
寡糖结构
β-agarase Polysiphonia urceolata Charged agarooligomer Neoagarotetraose Neoagarohexaose