改良二袖套法大鼠原位肝移植模型的建立

Establishment of an animal model of orthotopic liver transplantation with modified technique in rats

目的:探讨改良二袖套法大鼠原位肝移植模型的建立。方法:在Kamada等的袖套法吻合血管的基础上进行改进,供体门静脉进行肝脏灌注,肝上下腔静脉用缝合法吻合,门静脉和肝下下腔静脉用袖套法吻合,胆总管采用单管内支架胆管端端吻合下进行原位肝移植。结果:供体手术时间34±5.5 min;袖套准备12±6 min;受体手术时间42±6 min;无肝期22±4 min。手术后24 h内死亡4例,因为肝上下腔静脉出血2例,肝下下腔静脉出血1例,门静脉出血1例。手术成功率为93.3%。1 w生存率为88.5%。结论:改进的大鼠原位肝移植术操作简便,手术成功率高,可作为肝移植实验可靠、稳定的动物模型。

Objective： To discuss a new modified operational method for orthotopic liver transplantation with two - cuff technique. Methods. On the basis of cuff technique devised by Kamada, the donor liver was perfused with 10 to 15 ml cold（4℃） perfusate through portal vein（PV）. The suprahepatic vena cave（SVC） was anastomosed end to end with 8. 0 nylon line, the continuity of infrahepatic vena cave（IVC） and portal vein were established by means of cuff method, respectively. The bile duct anastomosis was performed by internal stent. Results： orthotopic liver transplantation was performed in 220 rats. The time for donor operation and cuff preparation was 34±5. 5 min and 12±6 min, and the recipient operative and anhepatic time was 42±6 min and 22±4 min, respectively. The one - day survival rate of post- operation was 93. 3%, one - week survival rate was 88. 5%. Conclusion： This modified model is feasible and highly reproducible and can become a practical and stable experimental model for the study of liver transplantation.