高羊茅胚性愈伤组织的高效诱导及其耐盐突变体筛选

High-efficiency inducement and salt-tolerant mutant selection of embryonic calli of Festuca arandinacea

以高羊茅Fine-lawn种子和下胚轴为外植体,分别在诱导培养基D1、D3、D5和D9上培养.下胚轴愈伤组织的出愈率除了在D1培养基上为51.3%外,在其他诱导培养基上均为100%.在D5培养基上继代3个月左右,从下胚轴获得了胚性愈伤组织.取在NaCl浓度为0.5%～3.0%的培养基上生长13 d的高羊茅胚性愈伤组织,分别测定其存活率、相对生长量、鲜干重比和脯氨酸含量.利用直接筛选的方法,将胚性愈伤组织分别放在含1%, 2%和3% NaCl的选择培养基上连续筛选60 d,在1% NaCl浓度下获得了耐盐的胚性愈伤组织并且获得了再生植株.再生植株能够在含有1% NaCl的Hoagland培养液中生长.

Calli was induced from seeds and hypocotyls of Festuca arundinacea fine-lawn on the callus-induction mediums D1, D3 , D5 , and D9 , respectively. The inducing rates of calli from the hypocotyls were 100 % on all mediums, except for D1 which had frequency of 51.7%. The embryonic calli were produced from callus-induction medium D5 after 3 months in the subculture. Survival frequency, relative growth rate, ratio of fresh weight to dry weight and proline content were measured 13 d after the calli of F. arandinacea were first cultured on a medium containing 0.5%-3.0% NaC1. The embryogenic calli were selected on a medium containing 1.0%, 2.0%, 3.0% NaC1 and directly continued for a further 60 d. Salt-tolerant variant types of calli were obtained from the medium containing 1.0% NaCl. Regenerated plants from these calli were shown to be able to survive in Hoagland solution with 1.0% NaCl.