摘要
目的:研究缺血预处理加川芎嗪对肠缺血再灌注大鼠心、肺细胞凋亡及相关基因B cl 2和B ax蛋白表达的影响。方法:健康SD大鼠,随机分为假手术对照组(Ⅰ组)、肠缺血再灌注组(Ⅱ组)、川芎嗪治疗组(Ⅲ组)、缺血预处理组(Ⅳ组)和川芎嗪+缺血预处理组(Ⅴ组)5组。用免疫组化法检测心、肺组织B cl 2和B ax蛋白表达,用末端脱氧核苷酸转移酶介导的生物素化脱氧尿嘧啶缺刻标记技术(TUNEL)检测心、肺细胞凋亡,并测定心、肺组织超氧化物歧化酶(SOD)和髓过氧化物酶(M PO)活性及丙二醛(M DA)含量。结果:Ⅴ组心、肺组织细胞凋亡指数明显低于除Ⅰ组外的其他各组,B cl 2蛋白表达明显增多,肺组织B ax蛋白表达明显减少,心肌细胞B ax蛋白表达明显比Ⅱ组降低,同时Ⅴ组心、肺组织SOD活性增高,M PO活性和M DA含量降低。结论:缺血预处理与川芎嗪联合应用对抑制肠缺血再灌注所致的心、肺细胞凋亡具有显著的协同作用。
Objective： To investigate the effect of ischemic preconditioning （IPC） and tetramethyl- pyrazine on apoptosis in heart and lung tissues and on the expression of cell apoptosis - associated gene bcl - 2 and bax after intestinal ischemia/reperfusion. Methods.. SD rats were randomly divided into sham operated control group （group Ⅰ ）, intestinal ischemia/reperfusion group （group Ⅱ ）, tetramethylpyrazine group（group Ⅲ ）, IPC group （group Ⅳ ） and IPC＋ tetramethylpyrazine group （group Ⅴ ）. Expression of Bcl - 2 and Bax protein in heart and lung tissues were detected by immunohistochemistry, and apoptosis of cells in heart and lung tissues were detected by terminal deoxynucleotidyl -transferase mediated dUTP -biotin nick end labeling （TUNEL）. The activities of superoxide dismutase （SOD） and myeloperoxidase （MPO） and the content of malondialdehyde （MDA） in heart and lung tissues were determined. Results： The combined use of tetramethylpyrazine and IPC greatly decreased apoptotic cells, the expression of Bax and the activities of MPO and levels of MDA, and enhanced the expression of Bcl - 2 and SOD activity. Conclusion ： The combined use of tetramethylpyrazine and IPC shows obvious synergistic effect on inhibiting apoptosis in heart and lung tissues after intestinal ischemia/reperfusion.
出处
《中国中西医结合急救杂志》
CAS
2005年第6期351-354,共4页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
浙江省中医药科研基金资助项目(2004C124)
浙江省温州市科技局科研基金资助项目(S2001A09)
关键词
缺血预处理
川芎嗪
细胞凋亡
缺血-再灌注损伤
肠
ischemic preconditioning
tetramethylpyrazine
apoptosis
intestine ischemia/reperfusion
