黄芪对感染性休克大鼠保护作用的实验研究

Experimental study on protective effect of astragalus on septic shock in rats

目的:探讨黄芪对感染性休克的作用及其机制.方法:健康成年雄性SD大鼠40只,采用盲肠结扎穿孔术(CLP)制备感染性休克模型.将动物随机均分为假手术组、模型组、黄芪低剂量组和黄芪高剂量组.行CLP前30 min黄芪组经静脉分别注入黄芪注射液10 g/kg和20 g/kg,假手术组和模型组大鼠输注等体积生理盐水(5 ml/kg).CLP后2～20 h每隔2 h经右股动脉测平均动脉压(MAP)和心率(HR);CLP后2、5、9和20 h采血测肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6).结果:模型组动物CLP后MAP进行性下降,HR先快后慢;两个黄芪组均能逆转CLP后MAP下降,HR波动小.模型组TNF-α水平于术后2 h达峰值;IL-6呈先升高后降低的趋势,于9 h达峰值:术后2、5和9 h,假手术组和两个黄芪组的TNF-α浓度均明显低于模型组(P均<0.01);术后5、9和20 h点,假手术组和两个黄芪组IL-6水平均明显低于模型组(P<0.05或P<0.01);黄芪高剂量组TNF-α于术后2 h、IL-6于术后9 h均显著低于黄芪低剂量组(P均<0.05).结论:黄芪通过抑制促炎性细胞因子TNF-α和IL-6的产生,对感染性休克大鼠具有明显的保护作用.

Objective： To investigate the effect of astragalus on rats with septic shock and its mechanism. Methods： Forty male SD rats were randomly divided into sham -operated group, model group, low-dose and high -dose astragalus groups. Septic shock model was induced by cecal ligation puncture （CLP）. Thirty minutes before CLP 10 g/kg or 20 g/kg of Huangqi injection （黄芪注射液）was injected intravenously in low -dose and high -dose astragalus groups respectively. Sham -operated and model groups received equal volume of saline （5 ml/kg）. Every 2 hours during 2 - 20 hours after CLP, the mean artery pressure （MAP） and heart rate （HR） were monitored via the right femoral artery. Tumor necrosis factor -α （TNF -α） and interleukin - 6 （IL - 6） in plasma were measured at 2, 5, 9 and 20 hours after CLP. Results： MAP decreased progressively after CLP in rats of model group, while HR ascended at first and descended afterwards. The decrease of MAP was inhibited, and the changes of HR were slight in the two astragalus groups. In the model group, TNF - α levels in plasma peaked at 2 hours after CLP, IL - 6 levels increased firstly and then decreased, peaking at 9 hours. TNF -α level was lower in sham -operated group and the two astragalus treatment groups than those in the model group at 2, 5 and 9 hours after CLP while IL - 6 at 5, 9 and 20 hours after CLP （P〈0. 05 or P〈0. 01）, and TNF - α level was lower in high - dose astragalus group than that in low- dose astragalus group at 2 hours while IL- 6 at 9 hours after CLP （both P〈0.05）. Conclusion： Astragalus has protective effects on septic shock rats by inhibiting the production of pro inflammatory cytokine TNF - α and IL - 6.