摘要
目的:建立简便、灵敏、准确的牛乳中氯霉素残留测定方法.方法:以非那西丁为内标,牛乳样品先采用10%高氯酸溶液沉淀蛋白,再经strata-X固相小柱萃取,洗脱液浓缩后在ODS(4.6×150 mm)柱上,以乙腈-5 mmol/L磷酸氢二铵缓冲溶液(22:78)为流动相,277 nm检测.结果:线性关系良好(r=0.9996),定量限(LOQ)为0.5 ng/ml,各浓度绝对回收率67.9%～77.6%,相对回收率85.4%～104.1%.结论:该方法简单、迅速、灵敏度高,适合用于牛乳中氯霉素的残留量的检测.
Objective：To develop a method for determination of residue of chloramphenicol in bovine milk. Methods：The milk, containing phenacetin as internal standard, is deproteined with 10% HClO4 first. The supernatant is then extracted and condensed with strata - X column. The target elution is determined on ODS （6.0 × 150 mm） column with acetonitrile -5 mmol/L diammonium hydrogen phosphate （22：78 ） as mobile phase and with UV detector being set at 277 nm. Results：The calibration curve is linear over the range of 0. 532 - 10. 64 ng/ml. The limit of quantification was 0. 532 ng/ml. Extraction recovery and the method recovery were 67. 9% - 77. 6% and 85.4% - 104. 1% respectively. Conclusion：The method is sensitive, accurate and convenient and reliable to determinate the residue of chloramphenicol in bovine milk.
出处
《中国卫生检验杂志》
CAS
2005年第12期1426-1428,共3页
Chinese Journal of Health Laboratory Technology
基金
2004年中国药科大学青年教师科技基金项目(A0304)
