九江市HIV-流行毒株基因序列测定和亚型分析

Subtype and sequence analysis of HIV-1 strains in Jiujiang

目的:了解九江市H IV-1流行毒株的亚型分布情况。方法:对采集的6份九江市H IV-1感染者的血浆样品进行RT-PCR扩增,获得GAG基因的核酸片断进行核苷酸序列分析。结果:在6份样品中发现B′亚型H IV-1毒株四株,B亚型和A/E重组亚型H IV-1毒株各一株。结论:H IV-1流行毒株亚型在九江市分布情况复杂,防控形势严峻。

Objective： To study the epidemic situation and characteristic of subtypes of HIV - 1 strains prevalent in Jiujiangy Methods：Six DNA fragments of HIV - 1 GAG gene were amplified by RT - PCR from blood plasma obtained from six HIV - 1 carrier in Jiujiang The genes were sequenced and analyzed. Results：There were four strains of HIV - 1 subtype B＇, one strain of HIV - 1 subtype B and one strain of HIV - 1 recombining subtype A/E. Conclusion：There is complex epidemic situation of subtypes of HIV - 1 strains prevalent in Jiujiang. The situation of HIV control and prevention is austere.