2002-2004年广西食源性致病菌监测研究

Surveillance for Food-borne Pathogens in Guangxi During the Period of 2002～2004

目的监测了解主要食品中致病菌污染状况,构建广西食品致病菌污染数据库,为有效防治食源性疾病提供科学依据.方法在南宁、桂林等6个市采集6大类食品,按国家标准或国际标准方法对食品中沙门氏菌、单核细胞增生李斯特菌(L.m)、大肠杆菌O157:H7、志贺氏菌以及海产品中的副溶血弧菌进行分离与鉴定.结果2002～2004年在6市5类食品中检出沙门氏菌54株,检出率4.49%;其次为检出单增细胞李斯特菌51株,检出率为4.24%,志贺氏菌为1.64%、大肠杆菌O157:H7为0 25%;而另1类食品海产品中副溶血检出率高达20.00%(41/205).结论广西主要食品均遭受食源性致病菌不同程度的污染;其中以生肉污染最为严重,受到了沙门氏菌、L.m、O157:H7、志贺氏菌的污染,熟肉、水产品、生食蔬菜也遭受L.m污染;而海产品副溶血弧菌污染严重.有针对性防范和控制各类食品致病菌的污染,是防止食源性疾病暴发的重要措施.

Objective To characterize the pathogens contamination in foods and develop a food-borne pathogens database of Guangxi as an evidence based effective prevention program for fight against the food - borne diseases. Methods Food samples were collected in 6 municipal cities and examined for presence of Salmonella spp., Listeria monocytogenes, Escherichia coli O157：H7, Shigella spp. and Vibrio parahaemolyticus according to the national or international standard guidelines. Results During2002-2004, 54（4.49%） salmonella isolates, 51（4.24%） Listeriamonocytogenes, 14（1.64%） Shigella and 3 （0.25 % ） Escherichia coli O157：H7, were identified. Among the 205 seafood samples, 41 （20.00 % ） V. parahaemolyticus strains were isolated. Conclusion Pathogens contaminated foods in Guangxi are frequent. Notably, raw meat is heavily contaminated by Salmonella spp., Listeria monocytogenes, Escherichia coli O157： HT, and Shigella spp.. Cooked meat, aquatic products and vegetable are frequently contaminated by L. monocytogenes, and seafood by V. parahaemolyticus. Specific efforts towards prevention controls of food contamination are crucial to prevent food- borne illness.