利用基因芯片技术研究乌头碱抗KBV_(200)细胞耐药机制

Using Gene Chip Technology to Investigate the Mechanism of Aconitine's Reversing the Drug Resistance of the KBV_(200) Cell

目的采用基因芯片技术研究乌头碱抗KBV200细胞耐药机制。方法提取乌头碱12.5μg/mL用药组和对照组KBV200细胞的RNA,进行cDNA微阵列分析。结果整张基因芯片5504个基因克隆,其中用药前高表达基因208个,占克隆总数的3.8%,用药后高表达基因196个,占3.6%。用药前后细胞凋亡相关基因、CDKS家族、SMADS家族、MAPK信号转导系统等的基因发生变化。结论乌头碱可能通过影响细胞凋亡相关基因和影响MAPK信号转导系统等机制,最后作用于Mdr1基因的表达,从而起到抗耐药作用。

Objective To investigate further the influence of aconitine to KBv200 related gene on the base of that it had been verified that degrading the expression of Pgp protein is the mechanism of aconitine＇s reversing the drug resistance of the KBv200 cell by using flow cytometry and immunohistochemical methods. Methods Extracted the RNA of KBv200 cell of both treat group （treated with 25 μg/mL aconitine） and control group, and carried out micro array analysis. Results There were total 5504 genes clones in the gene chip. The fluorescence signal scan showed that there were 208 genes with high expression （3.8%） before treating and 196 genes with high expression （3.6%） after treating. The gene of CDKS family, apoptosis related gene, SMADS family and MAPK signal transduction system changed after treating. Conclusion Aconitine may change the expression of Mdrl gene probably by influencing apoptosis related gene and MAPK signal transduction system and reverse drug resistance at last.