磁性CS-M固定HRP的条件研究

Immobilization of Horseradish Peroxidase on Magnetic Chitosan Microspheres

用磁性壳聚糖微球(磁性CS-M)作载体,用吸附法对辣根过氧化物酶(horseradishperoxidase,简称HRP)进行固定化研究。实验结果表明,磁性固定化酶的活力受壳聚糖浓度、交联剂的用量、乳化剂、载体与酶的比例、pH及固定化时间等因素的影响。

As a carder, the immobilization of Horseradish Peroxidase （HRP）on magnetic chitosan microspheres （Magnetic CS-M） was studied. The main immobilizing method was adsorption.The results showed that the activity of magnetic CS-M immobilizing HRP was affected by the concentration of chitosan, quantity of cross linker, Span-80, enzyme quantity, pH and the immobilizing time.