高压氧对精索静脉曲张家兔生殖激素水平影响的实验研究

Effect of Hyperbaric Oxygenation on the Reproductive Hormones of Rabbits with Varicocele

目的研究高压氧(HBO)对精索静脉曲张(VC)家兔生殖激素水平的影响,进一步探讨精索静脉曲张致男性不育的机制。方法采用HBO干预精索静脉曲张家兔模型,对睾丸重量、体积,精液参数及外周血浆生殖激素(FSH、LH)水平进行研究。结果与VC模型组比较,HBO干预VC模型组精液质量改善,睾丸重量增加,血浆FSH、LH水平下降(P=0.0215,P=0.0492),T水平增高(P<0·0001)。结论VC可导致睾丸间质损害,并引起生殖激素水平平衡失调,从而加重睾丸生精功能及附睾精子成熟功能的损害。主要机制为VC时睾丸缺血,缺氧及其微循环灌注障碍;HBO可有效抑制VC致睾丸间质细胞损害进程,改善睾丸间质细胞生存的微循环,维持生殖内分泌激素水平平衡。

Objective： To study the effect of hyperbaric oxygenation （HBO） on reproductive hormone of rabbits with varicocele（VC） and explore further mechanism of male infertility caused by varicocele. Methods： Thirty mature male rabbits were randomly divided into three group： pseudo- operation（A） , VC model（B） and VC model administered by HBO（C）. Serum hormones （FSH、LH） levels, testis volume and weight and parameters of seminal fluids were examined. Results： Comparing with those in group B, there was better sperm quality, heavier testicles and lower level of serum FSH and LH （P = 0.0215,P = 0. 0492）and higher level of T in group C （ Zhangjuan ： please fingure out what is T）. Conclusion ： Varicocele is harmful for interstitial tissue of testis, and it causes reproductive hormone level maladjusted. While HBO can restrain interstitial tissue of testis damaged by VC, improve their micro - circulation and balance level of reproductive hormone.